User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/10/05: Difference between revisions

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==Description==
==Description==
# 5 stock solutions, labeled A through E, were made by diluting MBP.  The initial concentration of the MBP was determined to be 10.714 μM.
# Solution A was made by mixing 1397 μL of 10.714 μM MBP with 1603 μL of 50 mM Tris buffer (pH 7.55).  The final concentration of solution A was 4.989 μM.
# Solution B was made by mixing 300 μL of solution A with 2700 μL of 50 mM Tris buffer (ph 7.55) for a final concentration of 0.4989 μM.
# Solution C was made by mixing 300 μL of solution B with 2700 μL of the same Tris buffer.  The final concentration was 49.89 nM.
# Solution D was made by mixing 300 μL of solution C with 2700 μL of the Tris buffer for a concentration of 4.989 nM.
# Solution E was made by mixing 300 μL of solution D with 2700 μL of Tris buffer to give a final concentration of 0.4989 nM.
# The intensity of each solution was taken from 310 - 500 nm when excited at 290 nm.


==Data==
==Data==

Revision as of 11:07, 19 October 2011

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Objective

To determine the fluorescence of MBP as a function of the concentration and to purify the DNA made from previously cultured cells.

Description

  1. 5 stock solutions, labeled A through E, were made by diluting MBP. The initial concentration of the MBP was determined to be 10.714 μM.
  2. Solution A was made by mixing 1397 μL of 10.714 μM MBP with 1603 μL of 50 mM Tris buffer (pH 7.55). The final concentration of solution A was 4.989 μM.
  3. Solution B was made by mixing 300 μL of solution A with 2700 μL of 50 mM Tris buffer (ph 7.55) for a final concentration of 0.4989 μM.
  4. Solution C was made by mixing 300 μL of solution B with 2700 μL of the same Tris buffer. The final concentration was 49.89 nM.
  5. Solution D was made by mixing 300 μL of solution C with 2700 μL of the Tris buffer for a concentration of 4.989 nM.
  6. Solution E was made by mixing 300 μL of solution D with 2700 μL of Tris buffer to give a final concentration of 0.4989 nM.
  7. The intensity of each solution was taken from 310 - 500 nm when excited at 290 nm.

Data

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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