User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/08/31: Difference between revisions

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==Description==
==Description==


# A 10 mL aqueous mixture of BSA (0.0015 mM) and HAuCl<sub>4</sub> (0.25 mM) was created using a 50 mM acetate buffer with a pH of 4.3.
# A 10 mL aqueous mixture of 0.001 g BSA (0.0015 mM) and 0.001 g HAuCl<sub>4</sub> (0.25 mM) was created using a 50 mM acetate buffer with a pH of 4.3.
# The reaction tube was then heated at 80°C for ______
# The reaction tube was then heated at 80°C for ______
# Every 30 minutes the UV-vis Spectrometer was used to assess the progress of the reaction.  Measurements were also taken of the buffer and the reaction mixture at the beginning of the reaction.  A wavelength range between 200 and 900 nm was used.  
# Every 30 minutes the UV-vis Spectrometer was used to assess the progress of the reaction.  Measurements were also taken of the buffer and the reaction mixture at the beginning of the reaction.  A wavelength range between 200 and 900 nm was used.


==Data==
==Data==

Revision as of 10:19, 31 August 2011

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Objective

To synthesize gold nanoparticles (AuNPs) from bovine serum albumen (BSA) and HAuCl4

Description

  1. A 10 mL aqueous mixture of 0.001 g BSA (0.0015 mM) and 0.001 g HAuCl4 (0.25 mM) was created using a 50 mM acetate buffer with a pH of 4.3.
  2. The reaction tube was then heated at 80°C for ______
  3. Every 30 minutes the UV-vis Spectrometer was used to assess the progress of the reaction. Measurements were also taken of the buffer and the reaction mixture at the beginning of the reaction. A wavelength range between 200 and 900 nm was used.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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