User:Eleni N. Kalivas/Notebook/CHEM-571/2014/10/01: Difference between revisions

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(Autocreate 2014/10/01 Entry for User:Eleni_N._Kalivas/Notebook/CHEM-571)
 
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==Entry title==
===Objective===
* Insert content here...
#Make stock concentrations (one set for both groups)
#Take UV-Vis and Fluorescence spectra for each stock
#Make a calibration curve for both fluorescence and UV-Vis
 
===Procedure===
====Preparation of stock solutions====
0.0120g of R6G in 10mL needed to make the initial stock solution of 2500uM, this was used to prepare a secondary stock of 500uM, used to make the dilutions for 0.1, 0.5, 0.75, 1.0, 1.2 and 1.5. The initial stock of 2500uM was used to make a 2uM. But the 1.5 and 2 uM were not used for the calibration curve because the fluorescence was too high that it did not provide accurate data.
 
*table of stock solution preparation
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration of stock solution (M)'''
| align="center" style="background:#f0f0f0;"|'''Amount of Stock used (mL)'''
|-
| 0.1||0.001
|-
| 0.5||0.005
|-
| 0.75||0.0075
|-
| 1||0.01
|-
| 1.2||0.012
|}
 
====Fluorescence====
*Parameters of Fluorimeter:
**500nm excitation
**515-700nm scan range
**10.0nm slit width
**200nm/min scan speed
<br>
*Fluorescence spectra
 
===Calibration curve based on Fluorescence===
 
====UV-Vis====
*Fluorescence Spectra
 
 




Revision as of 09:44, 3 October 2014

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Objective

  1. Make stock concentrations (one set for both groups)
  2. Take UV-Vis and Fluorescence spectra for each stock
  3. Make a calibration curve for both fluorescence and UV-Vis

Procedure

Preparation of stock solutions

0.0120g of R6G in 10mL needed to make the initial stock solution of 2500uM, this was used to prepare a secondary stock of 500uM, used to make the dilutions for 0.1, 0.5, 0.75, 1.0, 1.2 and 1.5. The initial stock of 2500uM was used to make a 2uM. But the 1.5 and 2 uM were not used for the calibration curve because the fluorescence was too high that it did not provide accurate data.

  • table of stock solution preparation
Concentration of stock solution (M) Amount of Stock used (mL)
0.1 0.001
0.5 0.005
0.75 0.0075
1 0.01
1.2 0.012

Fluorescence

  • Parameters of Fluorimeter:
    • 500nm excitation
    • 515-700nm scan range
    • 10.0nm slit width
    • 200nm/min scan speed


  • Fluorescence spectra

Calibration curve based on Fluorescence

UV-Vis

  • Fluorescence Spectra




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