User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/09: Difference between revisions
From OpenWetWare
(→Data) |
|||
(3 intermediate revisions by the same user not shown) | |||
Line 26: | Line 26: | ||
### Click accept | ### Click accept | ||
### Just before 1 minute add 30ul of 1.1u/mL ADA | ### Just before 1 minute add 30ul of 1.1u/mL ADA | ||
*NOTES: (already prepared by [[user:Matt Hartings|Matt Hartings]]) | |||
EHNA stock | |||
5mg EHNA in 1mL DMSO ---> 15.9mM EHNA | |||
(1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA | |||
The reaction samples will contain roughly 1nM EHNA | |||
==Data== | ==Data== | ||
* Graph of the changes in concentration from adenosine into inosine over time. | |||
[[Image:Adenosine Concentrations vs. Time.png|750 px]] | |||
*Adding EHNA appears to slow down the conversion of adenosine into inosine | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 12:16, 16 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveThe objective of today's lab is to observe and measure ADA turnover kinetics in the presence of, EHNA, an inhibitor. ProtocolThe following protocol was taken from Matt Harting's notebook
EHNA stock 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA (1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA The reaction samples will contain roughly 1nM EHNA Data
|