Methods
- Make 2 solutions of telomerase:
- Primers only (no AuNP):
- Mix 6.5 μL 100x diluted primers, 1 μL dNTP, 2.5 μL PCR buffer, and 250 μL diluted cell extract containing 100,000 cells.
- AuNP/TS primers:
- Mix 3 μL AuNP/TS primers, 1 μL dNTP, 2.5 μL PCR buffer, and 250 μL diluted cell extract containing 100,000 cells.
- Make a solution containing 260 μL tris buffer.
- Make a solution containing 250 μL diluted cell extract containing 100,000 cells, and 10 μL tris buffer.
- Incubate all solutions at room temperature for 30 min.
- Add 1.22 μL MB to all solutions.
- Incubate all solutions at 37oC for 1 hr.
- Take fluorescence data.
- Take fluorescence of dilutions of fluorescein solution.
Data
- Fluorescence of MB and cells:
- Fluorescence emission spectra for fluorescein dilutions, excited at 470 nm:
- Charts of relative fluorescence intensity:
- Peak fluorescence vs MB concentration for components of telomerase solution mixed with MB:
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