User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/08/25: Difference between revisions
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Revision as of 08:46, 27 August 2011
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High Stability Plasmid DevelopmentAbstract
InactivationsDouble digestions (DD) were inactivated for twenty minutes at 65°C, as indicated by the enzyme manuals.
Corroboration gelBoth reactions and their controls were loaded on the same gel:
LigationI prepared two ligation reactions, with plasmid-insert mass concentration ratios of 1:5 and 3:3.
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