User:Easilva/Notebook/Wayne/Entry Base: Difference between revisions
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* T75 (60% confluent HMVEC-D) flask was labeled with Live/Dead - Protocol: Cells were washed twice with PBS; 5 ul of both working solution of Calcein and Ethidium (2 uM and 4uM) were added to 5 mL of PBS; Cells were incubated for 45 min with 3 ml [from the solution] (plus fresh 7 mL of PBS) of labeling solution; washed twice with PBS and fresh media was used; | * T75 (60% confluent HMVEC-D) flask was labeled with Live/Dead - Protocol: Cells were washed twice with PBS; 5 ul of both working solution of Calcein and Ethidium (2 uM and 4uM) were added to 5 mL of PBS; Cells were incubated for 45 min with 3 ml [from the solution] (plus fresh 7 mL of PBS) of labeling solution; washed twice with PBS and fresh media was used; | ||
* Cells were visualized under the microscope and pictures taken and stored in the microscope computer under wayne folder; | * Cells were visualized under the microscope and pictures taken and stored in the microscope computer under wayne folder; | ||
* Still not clear and relatively weak signal from ethidium, could happen that cells are just very viable... | |||
Revision as of 21:11, 17 February 2013
Checking efficiency of Live/Dead staining | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
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