User:Eamonn B. Mallon/Notebook/Monoparental expression in worker reproduction genes/2010/06/14: Difference between revisions
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==Monoparental expression in worker reproduction genes== | ==Monoparental expression in worker reproduction genes== | ||
Basic protocol for production of reproducing workers. | |||
Mark callows from colony A. Put back in nest for 2 days. | |||
Once 2 days old add one worker from colony A plus two callows from B plus some young cocoons. | |||
Leave for 7 days. Older worker can assumed to be egg laying. Verify with pictures taken on addition and at 7 days (check that the brood pile has been added to. | |||
Store all bees at -80oC | |||
Revision as of 06:29, 14 June 2010
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Monoparental expression in worker reproduction genesBasic protocol for production of reproducing workers. Mark callows from colony A. Put back in nest for 2 days. Once 2 days old add one worker from colony A plus two callows from B plus some young cocoons. Leave for 7 days. Older worker can assumed to be egg laying. Verify with pictures taken on addition and at 7 days (check that the brood pile has been added to. Store all bees at -80oC
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