User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/09/30: Difference between revisions
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#Dialysis | #Dialysis | ||
#*Prepare 10 mL 0.5 g/L Lysozyme | #*Prepare 10 mL 0.5 g/L Lysozyme | ||
#*Prepare 2 mL 50 μM CaCl< | #*Prepare 2 mL 50 μM CaCl<sub>2</sub> (if you read ahead, you can use your CaCl<sub>2</sub> calibration stock) | ||
#*Prepare 2 mL 50 μM NaCl (if you read ahead, you can use your NaCl calibration stock) | #*Prepare 2 mL 50 μM NaCl (if you read ahead, you can use your NaCl calibration stock) | ||
#*Prepare 2 mL 50 μM KCl | #*Prepare 2 mL 50 μM KCl | ||
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#**You may need to tip the chamber on an angle to reduce water surface tension | #**You may need to tip the chamber on an angle to reduce water surface tension | ||
#*Fill the other side of the well with one of the following solutions | #*Fill the other side of the well with one of the following solutions | ||
#**HPLC water, 0.25 mM HCl, 50 μM CaCl< | #**HPLC water, 0.25 mM HCl, 50 μM CaCl<sub>2</sub>, 50 μM NaCl, or 50 μM KCl | ||
#**Each well has a different solution, which will be tested tomorrow | #**Each well has a different solution, which will be tested tomorrow | ||
#*Insert top screws to prevent leaking/evaporation | #*Insert top screws to prevent leaking/evaporation |
Revision as of 14:54, 29 September 2014
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TasklistWe will continue the rotation that we started last week.
All groups will set up dialysis.
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