User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/09/17: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Tasklist==
==Tasklist==
* Prepare SDS-PAGE [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24| Running-Buffer]
* Prepare SDS-PAGE [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 Running-Buffer]
* Run SDS-PAGE using this [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25| protocol]
* Run SDS-PAGE using this [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/25 protocol]
* While you wait during operation or staining, analyze your dialyzed solutions from yesterday
* While you wait during operation or staining, analyze your dialyzed solutions from yesterday
** Analyze both inside and outside the tubing
** Analyze both inside and outside the tubing

Latest revision as of 00:17, 27 September 2017

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Tasklist

  • Prepare SDS-PAGE Running-Buffer
  • Run SDS-PAGE using this protocol
  • While you wait during operation or staining, analyze your dialyzed solutions from yesterday
    • Analyze both inside and outside the tubing
    • Bradford analysis for protein content (be sure to analyze non-dialyzed AuNP - lysozyme colloidal solution)
    • pH and conductivity for salt and acid content
    • You may need to dilute the solution from the interior to have enough for a conductivity test


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