User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/09/16: Difference between revisions

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* Prepare 1 L 50 mM NaCl
* Prepare 1 L 50 mM NaCl
* Prepare 1 L 50 mM Glycine buffer, pH 3.5
* Prepare 1 L 50 mM Glycine buffer, pH 3.5
* Prepare 50 mL 1 g/L Lysozyme
* Prepare 25 mL 1 g/L Lysozyme
* Dialyze about 4 mL of lysozyme in about 3 in of dialysis tube
* Dialyze about 4 mL of lysozyme in about 3 in of dialysis tube
**Prepare 1 3500 g/mol MWCO tubing for NaCl, Glycine, & pure water soak
**Prepare 1 3500 g/mol MWCO tubing for NaCl, Glycine, & pure water soak

Revision as of 15:31, 15 September 2014

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Tasklist

  • If your AuNP colloid solution has precipitated, remake a 30:1 Au:protein (or lower Au) solution
  • Prepare 1 L 50 mM NaCl
  • Prepare 1 L 50 mM Glycine buffer, pH 3.5
  • Prepare 25 mL 1 g/L Lysozyme
  • Dialyze about 4 mL of lysozyme in about 3 in of dialysis tube
    • Prepare 1 3500 g/mol MWCO tubing for NaCl, Glycine, & pure water soak
    • Prepare 1 15k g/mol MWCO tubing for Glycine soak
    • Prepare 1 25k g/mol MWCO tubing for Glycine soak
  • Dialyze 4 mL of your colloid solution in 3500 g/mol MWCO tubing for Glycine soak



Details later tonight



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