User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/09/16: Difference between revisions
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== | ==Tasklist== | ||
* | * If your AuNP colloid solution has precipitated, remake a 30:1 Au:protein (or lower Au) solution | ||
* Prepare 1 L 50 mM NaCl | |||
* Prepare 1 L 50 mM Glycine buffer, pH 3.5 | |||
* Prepare 50 mL 1 g/L Lysozyme | |||
* Dialyze about 8 mL of lysozyme in 3 - 4 in of dialysis tube | |||
**Prepare 1 3500 g/mol MWCO tubing for NaCl, Glycine, & pure water soak | |||
**Prepare 1 15k g/mol MWCO tubing for Glycine soak | |||
**Prepare 1 25k g/mol MWCO tubing for Glycine soak | |||
*Dialyze 4 mL of your colloid solution in 3500 g/mol MWCO tubing for Glycine soak | |||
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Details later tonight | |||
Revision as of 15:30, 15 September 2014
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