User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/09/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Prepare 500 mL 50 mM NaCl | * Prepare 500 mL 50 mM NaCl | ||
* Prepare 1 L 50 mM Glycine buffer, pH 3.5 | * Prepare 1 L 50 mM Glycine buffer, pH 3.5 | ||
**As shown | **As shown in prior day notebook page, I used 0.4 mL of 1 M HCl to make 100 mL of buffer | ||
* Prepare 25 mL 1 g/L Lysozyme | * Prepare 25 mL 1 g/L Lysozyme | ||
* Dialyze about 4 mL of lysozyme in about 3 in of dialysis tube | * Dialyze about 4 mL of lysozyme in about 3 in of dialysis tube | ||
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*Analyze inside and outside dialysis membranes (pH, conductivity, Bradford) tomorrow while you wait for electrophoresis | *Analyze inside and outside dialysis membranes (pH, conductivity, Bradford) tomorrow while you wait for electrophoresis | ||
<br> | <br> | ||
==Dialysis Protocol== | ==Dialysis Protocol== | ||
#Cut about a 3" length of dialysis tubing | #Cut about a 3" length of dialysis tubing | ||
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#*we may use the shaker on low speed to help prevent gradients during dialysis | #*we may use the shaker on low speed to help prevent gradients during dialysis | ||
#*you should have 8 solutions when you are finished | #*you should have 8 solutions when you are finished | ||
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Latest revision as of 00:17, 27 September 2017
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Tasklist
Dialysis Protocol
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