User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/09/15

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Dialysis Test Prepare 100 mL Glycine Buffer with pH = 3.5 0.6 mL 0.95 M HCl + 0.37475 g glycine --> pH = 3.27 --> discarded 0.4 mL 0.95 M HCl + 0.37513 g glycine --> PH = 3.44 --> used with lysozyme 0.4 mL 0.95 M HCl + 0.37576 g glycine --> pH = 3.45 --> used with 30:1 AuNP - lysozyme Prepare 100 mL saline solution 0.9 wt-% = 150 mM NaCl 0.90066 g NaCl, 100.00xxx g total --> pH = 5.14 Prepare 90 mL 50 mM NaCl (by dilution) Prepare 25 mL 1.00 g/L lysozyme Prepare 4 mL 0.253 mM HAuCl4 (10x dilution of stock) --> pH = 3.41 Save portion of 50 mM dialysis solutions & lysozyme solution Transfer ~80 mL stock solution to a 400 mL plastic beaker Cut 3500 MWCO dialysis tubing and wet with DI water Clamp one end, add 10 mL of 1 g/L lysozyme, clamp other end & place in 50 mM NaCl Repeat, but place in 50 mM glycine buffer (in beaker) Repeat, but use ~9 mL 30:1 AuNP - lysozyme colloid (inside) & 50 mM glycine buffer (in beaker) one side loosened, xfer solution to test tube, reclamp and transfer back to tubing only about 6 - 7 mL left after xfers rinsed exterior with DI water prior to soak in buffer