User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/08/01: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/08/01 Entry for User:Douglas_M._Fox/Notebook/AU_CHEM-571_F2011_Lab_Support)
 
(fix raw html notebook nav)
 
(2 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==AuNP Synthesis==
* Insert content here...
#HAuCl<sub>4</sub> Stock Solution
#*42.97 mg HAuCl<sub>4</sub> using parafilmed spatula
#*50 mL water in vol flask
#*2.53 mM stock soln
#Volumes Needed For Colloid & Fiber Solutions
{| {{table}}
|-
! !! Lys Colloid !! Lys Fiber !! BSA Colloid !! BSA fiber
|-
| Au:protein || < 35 || > 35 || < 135 || > 135
|-
| [protein stock] || 70.96 μM ||  || 17.73 μM ||
|-
| Vol HCl || 988 μL || 988 μL || 988 μL || 988 μL
|-
| Vol protein || 1.174 mL || 0.881 mL || 1.083 mL || 1.066 mL
|-
| Au:protein || 30 || 40 || 130 || 140
|}
 
<ol start="3">
<li>AuNP Preparations<br />
*30:1 Au-Lys --> 988 μL Au + 2*587 μL protein + 7.838 mL H2O<br />
*40:1 Au-Lys --> 988 μL Au + 2*440 μL protein + 8.131 mL H2O<br />
*140:1 Au-BSA --> 988 μL Au + 2*503 μL protein + 8.006 mL H2O<br />
*Heated in foil covered glass test tubes 80 °C for 3 hr<br />
*Added 24.32 mg CaCl2 ("anhydrous" label, but visibly wet) to 30:1 Au-Lys solution<br />
*Fibers in other two, as expected<br />
*Invertase not dissolved, so not used</li>
</ol>




Latest revision as of 00:26, 27 September 2017

Project name Main project page
Previous entry      Next entry

AuNP Synthesis

  1. HAuCl4 Stock Solution
    • 42.97 mg HAuCl4 using parafilmed spatula
    • 50 mL water in vol flask
    • 2.53 mM stock soln
  2. Volumes Needed For Colloid & Fiber Solutions
Lys Colloid Lys Fiber BSA Colloid BSA fiber
Au:protein < 35 > 35 < 135 > 135
[protein stock] 70.96 μM 17.73 μM
Vol HCl 988 μL 988 μL 988 μL 988 μL
Vol protein 1.174 mL 0.881 mL 1.083 mL 1.066 mL
Au:protein 30 40 130 140
  1. AuNP Preparations
    • 30:1 Au-Lys --> 988 μL Au + 2*587 μL protein + 7.838 mL H2O
    • 40:1 Au-Lys --> 988 μL Au + 2*440 μL protein + 8.131 mL H2O
    • 140:1 Au-BSA --> 988 μL Au + 2*503 μL protein + 8.006 mL H2O
    • Heated in foil covered glass test tubes 80 °C for 3 hr
    • Added 24.32 mg CaCl2 ("anhydrous" label, but visibly wet) to 30:1 Au-Lys solution
    • Fibers in other two, as expected
    • Invertase not dissolved, so not used



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Douglas_M._Fox/Notebook/AU_CHEM-571_F2011_Lab_Support/2014/08/01&oldid=1015619"