Protein Solution Preparation
- Lysozyme
- Mw = 14,307 Da
- 0.00637 g in 25 mL vol flask (17.81 μM)
- 0.02538 g in 25 mL vol flask (70.96 μM = 1.01 g/L)
- BSA
- Mw = 66,430 Da
- 0.02944 g in 25 mL vol flask (17.73 μM = 1.18 g/L)
- Invertase
- Mw = 270,000 Da
- 0.09497 g in 10 mL vol flask (35.2 μM = 9.56 g/L)
- Did not completely dissolve
General Procedure for determining appropriate concentration:
- Identify [Au]:[protein] concentration required for fiber formation
- [Au]f = 0.25 mM = 250 μM for correct pH
- Want to add 1 mL of each stock, diluted to 10 mL for fiber formation
- [Au]stock = 2.5 mM
- For BSA, fiber formation occurs at [Au]:[protein] = 135
- [Protein] = [Au]/135 = 18.5 μM
- Note that the final prepared solution will have [protein]f = 1.85 μM
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