User:Dominique Brager/Notebook/481 Notebook/2016/10/19: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(9 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 14: Line 14:


==Description==
==Description==
* Prepare 10mL of 100ppm solutions of Pb, Zn, Cu  
* Prepare 100mL of 100ppm solutions of Pb, Zn, Cu  
:*(120 ppm solution prepared of Zn by Sarah)
:*Cu
::100mL*1L/1000mL*100mg Cu/1L*134.45 g/mol CuCl<sub>2</sub>/63.54 g/mol Cu = 21.16 mg CuCl<sub>2</sub>


*Cu
:*Pb
:10mL*1L/1000mL*100mg Cu/1L*134.45 g/mol CuCl<sub>2</sub>/63.54 g/mol Cu = 2.116 mg CuCl<sub>2</sub>
::100mL*1L/1000mL*100mg Pb/1L*325.29 g/mol PbAc/207.2 g/mol Cu = 15.7 mg PbAc


*Pb
:*Zn
:10mL*1L/1000mL*100mg Pb/1L*325.29 g/mol PbAc/207.2 g/mol Cu = 1.57 mg PbAc
::100mL*1L/1000mL*100mg Zn/1L*138.315 g/mol ZnCl<sub>2</sub>/65.38 g/mol Zn = 21.15 mg ZnCl<sub>2</sub>


*Zn
*Prepare 50mL of 300 ppm metals (100ppm Pb, 100ppm Zn, 100ppm Cu)
:10mL*1L/1000mL*120mg Zn/1L*138.315 g/mol ZnCl<sub>2</sub>/65.38 g/mol Zn = 2.66 mg ZnCl<sub>2</sub>
: 10.58 mg CuCl<sub>2</sub>
: 10.57 mg ZnCl<sub>2</sub>
: 7.85 mg PbAc
: 50mL H<sub>2</sub>O
 
:*Dilution to 60ppm
:: 10mL of 300ppm and 40mL water
 
*20% column data:
Dry:15.5904g
 
Wet: 15.9570g
 
W/Beads: 16.65g
 
*10% column data
Dry:15.966g
 
Wet: 16.389g
 
W/Beads: 17.55g
 
Ran data analysis on 10% beads with MG
* Found the Max Absorbance for each run.
*Using the Beer's Law calibration Curve we found a few weeks ago (y=0.0813x) calculated concentration for each sample.
*Created plot of concentration vs. Volume Eluted.
*Calculated the amount of MG added to the Column
::: 54 samples *5mL/sample*1L/1000mL*50mg/1L = 1.75mg
*Calculated the amount of MG eluted.
::: From samples 1-54:
:::: 5mL*1L/1000mL*concentration of sample = mg of MG not adsorbed
::: For sample 55:
:::: 1mL*1L/1000mL*concentration of sample = mg of MG not adsorbed
:::: 4mL*1L/1000mL*concentration of sample = mg of MG desorbed
::: For samples 56-88:
:::: 5mL*1L/1000mL*concentration of sample = mg of MG desorbed
*Subtract mg of MG not adsorbed from amount of MG added to column to get amount of MG adsorbed by the column.
*Find capacity of beads by dividing mass of MG adsorbed by mass of beads
*Find capacity of activated carbon by dividing mass of MG adsorbed by mass of activated carbon in beads.


==Data==
==Data==
* Add data and results here...
Data and Results for 10% Column with MG


==Notes==
Graph of Concentration of MG vs. Volume of MG Eluted
This area is for any observations or conclusions that you would like to note.
[[Image:10%graph2.png|700px]]




Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
Amount of MG loaded: 13.5mg


[[Category:Course]]
Amount not adsorbed: 0.6025mg
[[Category:Miscellaneous]]


Amount adsorbed: 13.5mg-0.6025mg=12.9mg


Amount desorbed: 0.2879mg


[[Category:Course]]
Mass of Wet Beads in Column: 1.465g
[[Category:Miscellaneous]]


Mass of Dry Beads in Column: 0.2683g


Capacity of Beads: 48.08 mg/g


Mass of Clay in Column: 0.02683g


Capacity of Clay: 480.8 mg/g


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}


__NOTOC__
Swelling for 10% clay is 446%
*4.46=wet-dry/dry
*4.46*dry = wet-dry
*4.46dry+dry= wet
*5.46dry = wet
*wet = 1.465g
*1.465/5.46 = dry
*dry mass = 0.2683g

Latest revision as of 02:05, 27 September 2017

Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

  • Prepare heavy metal solutions (Zn, Pb, Cu)

Description

  • Prepare 100mL of 100ppm solutions of Pb, Zn, Cu
  • Cu
100mL*1L/1000mL*100mg Cu/1L*134.45 g/mol CuCl2/63.54 g/mol Cu = 21.16 mg CuCl2
  • Pb
100mL*1L/1000mL*100mg Pb/1L*325.29 g/mol PbAc/207.2 g/mol Cu = 15.7 mg PbAc
  • Zn
100mL*1L/1000mL*100mg Zn/1L*138.315 g/mol ZnCl2/65.38 g/mol Zn = 21.15 mg ZnCl2
  • Prepare 50mL of 300 ppm metals (100ppm Pb, 100ppm Zn, 100ppm Cu)
10.58 mg CuCl2
10.57 mg ZnCl2
7.85 mg PbAc
50mL H2O
  • Dilution to 60ppm
10mL of 300ppm and 40mL water
  • 20% column data:

Dry:15.5904g

Wet: 15.9570g

W/Beads: 16.65g

  • 10% column data

Dry:15.966g

Wet: 16.389g

W/Beads: 17.55g

Ran data analysis on 10% beads with MG

  • Found the Max Absorbance for each run.
  • Using the Beer's Law calibration Curve we found a few weeks ago (y=0.0813x) calculated concentration for each sample.
  • Created plot of concentration vs. Volume Eluted.
  • Calculated the amount of MG added to the Column
54 samples *5mL/sample*1L/1000mL*50mg/1L = 1.75mg
  • Calculated the amount of MG eluted.
From samples 1-54:
5mL*1L/1000mL*concentration of sample = mg of MG not adsorbed
For sample 55:
1mL*1L/1000mL*concentration of sample = mg of MG not adsorbed
4mL*1L/1000mL*concentration of sample = mg of MG desorbed
For samples 56-88:
5mL*1L/1000mL*concentration of sample = mg of MG desorbed
  • Subtract mg of MG not adsorbed from amount of MG added to column to get amount of MG adsorbed by the column.
  • Find capacity of beads by dividing mass of MG adsorbed by mass of beads
  • Find capacity of activated carbon by dividing mass of MG adsorbed by mass of activated carbon in beads.

Data

Data and Results for 10% Column with MG

Graph of Concentration of MG vs. Volume of MG Eluted


Amount of MG loaded: 13.5mg

Amount not adsorbed: 0.6025mg

Amount adsorbed: 13.5mg-0.6025mg=12.9mg

Amount desorbed: 0.2879mg

Mass of Wet Beads in Column: 1.465g

Mass of Dry Beads in Column: 0.2683g

Capacity of Beads: 48.08 mg/g

Mass of Clay in Column: 0.02683g

Capacity of Clay: 480.8 mg/g


Swelling for 10% clay is 446%

  • 4.46=wet-dry/dry
  • 4.46*dry = wet-dry
  • 4.46dry+dry= wet
  • 5.46dry = wet
  • wet = 1.465g
  • 1.465/5.46 = dry
  • dry mass = 0.2683g
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Dominique_Brager/Notebook/481_Notebook/2016/10/19&oldid=1018689"