User:Dileep D. Monie/Recipes/Supplemented M9

Supplemented M9 Media (500 ml)

NOTE: This is the same as the Endy Lab version but does not require preparation of intermediate solutions

• Mix the following components, allow to dissolve completely, and sterilize with a 0.2 μm PES sterile filter
  1. 497 ml of ddH₂O
  2. 50 μl of 1M CaCl₂ (Teknova C0477)
  3. 1 ml of 1M MgSO₄ (Sigma M3409)
  4. 5.64 g of M9 minimal salts, 5X (Sigma M6030)
  5. 150 mg of thiamine hydrochloride (Sigma T1270)
  6. 1 g of casamino acids (Acros Organics 61204)
  7. 2 ml of glycerol (Invitrogen 15514)
• Protect Supplemented M9 Media from light and store at 4°C