User:Dileep D. Monie/Notebook/BBF Standards Measurement/2008/04/03: Difference between revisions

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(Autocreate 2008/04/03 Entry for User:Dileep_D._Monie/Notebook/BBF_Standards_Measurement)
 
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==Entry title==
==Entry title==
* Insert content here...
*Analyzed flow cytometry data collected on 2008-4-2 using BD CellQuest Pro v5.2
*Forward vs side scatter plot showing TOP10 cells (1% of events are shown) and R1 gate used for statistics
[[Image:DDM_TOP10-A.JPG]]
*Histogram overlay showing FL1 signals (GFP) of representative samples from each group (gated on R1) and markers (M1 and M2) used for statistics
[[Image:DDM_overlay.JPG]]
#Purple solid: TOP10
#Green line: I20259
#Pink line: I20269
#Dotted line: I20270
#Orange line: I20268
#Dark blue line: I20260
*Calculated geometric means of fluorescence intensities (I<sub>sample,MX</sub>) for triplicate samples (bounded by appropriate marker)
<pre>
TOP10 (M1) I20259 (M1) I20269 (M2) I20270 (M2) I20268 (M2) I20260 (M2)
1.62 1.66 34.62 78.93 133.87 170.4
1.65 1.68 33.44 77.06 133.29 168.55
1.65 1.67 34.15 77.18 137.02 171.03
</pre>
*Calculated fluorescence intensities in consensus BioBrick™ GFP units (I/I<sub>I20260</sub>) using (<I<sub>sample,MX</sub>>-<I<sub>TOP10,M1</sub>>)/(<I<sub>I20260,M2</sub>>-<I<sub>TOP10,M1</sub>>)
<pre>
TOP10 I20259 I20269 I20270 I20268 I20260
0 0.000178197 0.192630579 0.451926504 0.790519938 1
</pre>
[[Image:DDM_promoter_chart.jpg]]




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Revision as of 11:07, 8 April 2008

BBF Standards: Promoter Measurement Test <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Entry title

  • Analyzed flow cytometry data collected on 2008-4-2 using BD CellQuest Pro v5.2
  • Forward vs side scatter plot showing TOP10 cells (1% of events are shown) and R1 gate used for statistics

  • Histogram overlay showing FL1 signals (GFP) of representative samples from each group (gated on R1) and markers (M1 and M2) used for statistics

  1. Purple solid: TOP10
  2. Green line: I20259
  3. Pink line: I20269
  4. Dotted line: I20270
  5. Orange line: I20268
  6. Dark blue line: I20260
  • Calculated geometric means of fluorescence intensities (Isample,MX) for triplicate samples (bounded by appropriate marker)
TOP10 (M1)	I20259 (M1)	I20269 (M2)	I20270 (M2)	I20268 (M2)	I20260 (M2)
1.62		1.66		34.62		78.93		133.87		170.4
1.65		1.68		33.44		77.06		133.29		168.55
1.65		1.67		34.15		77.18		137.02		171.03
  • Calculated fluorescence intensities in consensus BioBrick™ GFP units (I/II20260) using (<Isample,MX>-<ITOP10,M1>)/(<II20260,M2>-<ITOP10,M1>)
TOP10	I20259		I20269		I20270		I20268		I20260
0	0.000178197	0.192630579	0.451926504	0.790519938	1


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