User:Dileep D. Monie/Notebook/BBF Standards Measurement/2008/03/31: Difference between revisions

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*Prepared 500 ml of Supplemented M9 Media (this is the same as the [[Endy:M9_media/supplemented|Endy Lab version]] but does not require preparation of intermediate solutions)
*Prepared 500 ml of Supplemented M9 Media (this is the same as the [[Endy:M9_media/supplemented|Endy Lab version]] but does not require preparation of intermediate solutions)
**Mix the following components, allow to dissolve completely, and sterilize with a 0.2 μm PES sterile filter
**Mix the following components, allow to dissolve completely, and sterilize with a 0.2 μm PES sterile filter
*#497 ml ddH<sub>2</sub>O
*#497 ml of ddH<sub>2</sub>O
*#50 μl 1M CaCl<sub>2</sub> (Teknova C0477; lot no. C047710L301Z)
*#50 μl of 1M CaCl<sub>2</sub> (Teknova C0477; lot no. C047710L301Z)
*#1 ml 1M MgSO<sub>4</sub> (Sigma M3409; lot no. 116K6095)
*#1 ml of 1M MgSO<sub>4</sub> (Sigma M3409; lot no. 116K6095)
*#5.64 g M9 minimal salts, 5X (Sigma M6030; lot no. 127K1574)
*#5.64 g of M9 minimal salts, 5X (Sigma M6030; lot no. 127K1574)
*#150 mg thiamine hydrochloride (Sigma T1270; lot no. 037K00071)
*#150 mg of thiamine hydrochloride (Sigma T1270; lot no. 037K00071)
*#1 g casamino acids (Acros 61204-1000; lot no. B0123863)
*#1 g of casamino acids (Acros 61204-1000; lot no. B0123863)
*#2 ml glycerol (Invitrogen 15514-011; lot no. 1248326)
*#2 ml of glycerol (Invitrogen 15514-011; lot no. 1248326)
**Protect Supplemented M9 Media from light and store at 4°C
**Protect Supplemented M9 Media from light and store at 4°C


Revision as of 19:32, 8 April 2008

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Entry title

  • Prepared 500 ml of Supplemented M9 Media (this is the same as the Endy Lab version but does not require preparation of intermediate solutions)
    • Mix the following components, allow to dissolve completely, and sterilize with a 0.2 μm PES sterile filter
    1. 497 ml of ddH2O
    2. 50 μl of 1M CaCl2 (Teknova C0477; lot no. C047710L301Z)
    3. 1 ml of 1M MgSO4 (Sigma M3409; lot no. 116K6095)
    4. 5.64 g of M9 minimal salts, 5X (Sigma M6030; lot no. 127K1574)
    5. 150 mg of thiamine hydrochloride (Sigma T1270; lot no. 037K00071)
    6. 1 g of casamino acids (Acros 61204-1000; lot no. B0123863)
    7. 2 ml of glycerol (Invitrogen 15514-011; lot no. 1248326)
    • Protect Supplemented M9 Media from light and store at 4°C


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