User:Dileep D. Monie/Notebook/BBF Standards Measurement/2008/03/31: Difference between revisions
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(Autocreate 2008/03/31 Entry for User:Dileep_D._Monie/Notebook/BBF_Standards_Measurement) |
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* | *Prepared 500 ml of Supplemented M9 Media (this is the same as the [[Endy:M9_media/supplemented|Endy Lab version]] but does not require preparation of intermediate solutions) | ||
**Mix the following components, allow to dissolve completely, and sterilize with a 0.2 μm PES sterile filter | |||
*#497 ml ddH<sub>2</sub>O | |||
*#50 μl 1M CaCl<sub>2</sub> (Teknova C0477; lot no. C047710L301Z) | |||
*#1 ml 1M MgSO<sub>4</sub> (Sigma M3409; lot no. 116K6095) | |||
*#5.64 g M9 minimal salts, 5X (Sigma M6030; lot no. 127K1574) | |||
*#150 mg thiamine hydrochloride (Sigma T1270; lot no. 037K00071) | |||
*#1 g casamino acids (Acros 61204-1000; lot no. B0123863) | |||
*#2 ml glycerol (Invitrogen 15514-011; lot no. 1248326) | |||
**Protect Supplemented M9 Media from light and store at 4°C | |||
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Revision as of 11:03, 8 April 2008
 BBF Standards: Promoter Measurement Test
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