User:Dileep D. Monie/Notebook/2008-3-27: Difference between revisions
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*#Dissolve 100 mg thiamine hydrochloride (Sigma T1270; lot no. 037K00071) in 10 ml ddH<sub>2</sub>O | *#Dissolve 100 mg thiamine hydrochloride (Sigma T1270; lot no. 037K00071) in 10 ml ddH<sub>2</sub>O | ||
*#Filter sterilize with a 0.2 μm PES sterile syringe filter | *#Filter sterilize with a 0.2 μm PES sterile syringe filter | ||
**Protect Supplemented M9 Media from light | **Protect Supplemented M9 Media from light and store at 4°C | ||
Revision as of 00:34, 29 March 2008
- Prepared 250 ml of Supplemented M9 Media (this is the same as the Endy Lab version but does not require preparation of intermediate solutions)
- Mix the following components and autoclave at 121°C for 20 min
- 241 ml ddH2O
- 2.82 g M9 minimal salts, 5X (Sigma M6030; lot no. 127K1574)
- Note corrected amount
- 0.5 g casamino acids (Acros 61204-1000; lot no. B0123863)
- 1 ml glycerol (Invitrogen 15514-011; lot no. 1248326)
- 0.5 ml 1M MgSO4 (Sigma M3409; lot no. 116K6095)
- 25 μl 1M CaCl2 (Teknova C0477; lot no. C047710L301Z)
- Add 7.5 ml of sterile 10 mg/ml thiamine hydrochloride solution
- Dissolve 100 mg thiamine hydrochloride (Sigma T1270; lot no. 037K00071) in 10 ml ddH2O
- Filter sterilize with a 0.2 μm PES sterile syringe filter
- Protect Supplemented M9 Media from light and store at 4°C
