User:Dileep D. Monie

Contact Info

• Dileep D. Monie
• Johns Hopkins University
• Baltimore, MD, USA
• Email me through OpenWetWare

I work in the Wilmer Eye Institute at the JHU School of Medicine. I learned about OpenWetWare from the 24C3 Endy lecture, and I've joined because I am interested in contributing to the BBF Technical Standards.

Research Interests

1. Synthetic Biology
   1. Standards - Establishing standards for biological parts to ensure interoperability and allow for construction of complex systems
   2. Biocircuits - Implementing wetware abstraction layers to allow creation of devices for cellular computation and communications
   3. Epigenetics - Manipulating chromatin structures with histone and DNA modifications as a form of biological software programming
   4. Delivery Systems - Making viruses or other vectors for targeted delivery of DNA parts into an established host genome
2. Bioengineering
   1. Immunology - Defining the theoretical framework and designing components for an artificial immune system
   2. Drug Discovery - Designing cell reporter systems to detect biomolecule interactions and perturbations in signal transduction
   3. Astrobiology - Engineering energy pathways and lifeforms sustainable in extreme environments
   4. Biocomputing - Integrating logic biocircuits on a very large scale in single cell and multicellular systems
3. Biosecurity and Biosafety
   1. Policy - Balancing the needs of an open hacker community with access restrictions
   2. Testing and Certification - Establishing an "Underwriters Laboratories" for developing standards and test procedures for biosafety
   3. Countermeasures - Designing fail-safe mechanisms to mitigate potential synthetic biology abuses

BBF Contributions

• Promoter Measurement Test

OWW Brain Dump

• Notebook
• Recipes
• Upload log

Publications

1. Monday SR, Vath GM, Ferens WA, Deobald C, Rago JV, Gahr PJ, Monie DD, Iandolo JJ, Chapes SK, Davis WC, Ohlendorf DH, Schlievert PM, and Bohach GA. Unique superantigen activity of staphylococcal exfoliative toxins. J Immunol. 1999 Apr 15;162(8):4550-9.
2. Vath GM, Earhart CA, Monie DD, Iandolo JJ, Schlievert PM, and Ohlendorf DH. The crystal structure of exfoliative toxin B: a superantigen with enzymatic activity. Biochemistry. 1999 Aug 10;38(32):10239-46. DOI:10.1021/bi990721e |
3. Kelly JR, Rubin AJ, Davis JH, Ajo-Franklin CM, Cumbers J, Czar MJ, de Mora K, Glieberman AL, Monie DD, and Endy D. Measuring the activity of BioBrick promoters using an in vivo reference standard. J Biol Eng. 2009 Mar 20;3:4. DOI:10.1186/1754-1611-3-4 |
4. ISBN:9780781764964

   Contributed section on "Pulmonary Hypertension" (pp. 148-9)