User:Dhea Patel/Notebook/Phosphorylation/2012/02/16: Difference between revisions
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==Description== | ==Description== | ||
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Preparation of y-phosphate ATP, as described by Haifeng Son, Kagan Kerman and Heinz-Bernhard Kraatz' ''Electronic Supplementary Information (ESI) for'' '''Electrochemical detection of kinase-catalysed phosphorylation using ferrocene-conjugated ATP''' | |||
# 1M TEAB Buffer (pH = 7.5) was prepared by filling a 1L Erlenmeyer flask with dry ice and filling a 2L Erlenmeyer flask with a side arm with 1 M aqueous solution of triethylamine. | |||
# Both Erlenmeyer flasks were corked and connected to each other through a tubing contraption that allowed the CO<sub>2</sub> from the dry ice to carbonate the triethylamine in the second flask. The excess gas formed in the second flask exited through the side arm. | |||
# The CO<sub>2</sub> bubbled until the TEA(aq) reached a pH of 7.5. | |||
"'Calculations for 1M Triethylamine solution'" | |||
(1M) × (101.19 g/mol) × (1/0.7255g/mL) × (L/1000mL) × 1.5 L = 0.209 L = 209 mL triethylamine to make 1.5 L solution | |||
1500 mL − 209 mL = 1291 mL distilled water | |||
==Data== | ==Data== | ||
Revision as of 09:31, 16 February 2012
 Phosphorylation Lab
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ObjectiveLearn how to maintain an OpenWetWare Notebook. DescriptionPreparation of y-phosphate ATP, as described by Haifeng Son, Kagan Kerman and Heinz-Bernhard Kraatz' Electronic Supplementary Information (ESI) for Electrochemical detection of kinase-catalysed phosphorylation using ferrocene-conjugated ATP
(1M) × (101.19 g/mol) × (1/0.7255g/mL) × (L/1000mL) × 1.5 L = 0.209 L = 209 mL triethylamine to make 1.5 L solution 1500 mL − 209 mL = 1291 mL distilled water Data
NotesThis area is for any observations or conclusions that you would like to note. |
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