User:Dhea Patel/Notebook/Hemoglobin Project/2014/02/05: Difference between revisions
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==OPD in H<sub>2</sub>O== | ==Setting Up Ocean Optics== | ||
*Run Wizard for Absorbance Spectroscopy | |||
*Configure Data Collection as described below | |||
*Set Reference Blank and Dark | |||
*On the Absorbance view, click the "save" button to configure Save Settings | |||
*When you are ready to collect data, place the cuvette in the holder (with the cap) and click the "play" arrow followed by the "save" button. | |||
==OPD in H<sub>2</sub>O at 35C== | |||
#Stock solutions: | #Stock solutions: | ||
| Line 18: | Line 26: | ||
##Sample chamber set to 35C and to stir | ##Sample chamber set to 35C and to stir | ||
##2.025mL OPD stock solution | ##2.025mL OPD stock solution | ||
##0.75mL | ##0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | ||
##Start Data Collection | ##Start Data Collection | ||
###1ms Integration | ###1ms Integration | ||
| Line 25: | Line 33: | ||
###Stop after 2 hours | ###Stop after 2 hours | ||
###Files saved as: OPD_H2O2_H2O_35_SCANNUMBER.txt | ###Files saved as: OPD_H2O2_H2O_35_SCANNUMBER.txt | ||
##0.225mL H2O added just before the third scan | |||
###This is denoted in the data file for the third scan | |||
==OPD in H<sub>2</sub>O at 45°C== | |||
#The stock solutions were remade: | |||
##3.3 mg of OPD in 3.3 mL of H<sub>2</sub>O | |||
##0.114 mL of 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL H<sub>2</sub>O | |||
#Experimental Run: | |||
##Sample chamber set to 45°C and stir | |||
##2.025mL OPD stock solution | |||
##0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
##Start Data Collection | |||
###1ms Integration | |||
###10 scan average | |||
###Wait 1 minute after each scan | |||
###Stop after 2 hours | |||
###Files saved as: OPD_H2O2_H2O_45_SCANNUMBER.txt | |||
##0.225mL H2O added just before the third scan | ##0.225mL H2O added just before the third scan | ||
###This is denoted in the data file for the third scan | ###This is denoted in the data file for the third scan | ||
Revision as of 10:28, 5 February 2014
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Setting Up Ocean Optics
OPD in H2O at 35C
OPD in H2O at 45°C
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