User:Dhea Patel/Notebook/Hemoglobin Project/2013/06/13: Difference between revisions
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== | ==Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb Catalyst in Acetone Suspension== | ||
The [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/31 procedure]for the reaction of OPD and H<sub>2</sub>O<sub>2</sub> was followed using a Mb/Tris catalyst and an acetone suspension. The reaction was run in a beaker with a stir bar and the solution was centrifuged for 1 minute at 13,200 rpm before taking a spectrum. | |||
In addition reactions were run using Mb/Phosphate and Mb/Acetate in hexanes suspension. | |||
These samples were filtered (not centrifuged) before taking a spectrum. | |||
==Data== | |||
<b>Mb/Tris in Acetone, Trial 1</b> | |||
[[Image:Mb_Tris1_OPD_H2O2_Acetone_WORKUP_GRAPH.JPG]] | |||
The drop in absorbance at 140 minutes is due to the outside of the cuvette being cleaned. | |||
<b>Mb/Tris in Acetone, Trial 2</b> | |||
[[]] | |||
The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned. | |||
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Reaction of OPD+H2O2 with Mb Catalyst in Acetone SuspensionThe procedurefor the reaction of OPD and H2O2 was followed using a Mb/Tris catalyst and an acetone suspension. The reaction was run in a beaker with a stir bar and the solution was centrifuged for 1 minute at 13,200 rpm before taking a spectrum. In addition reactions were run using Mb/Phosphate and Mb/Acetate in hexanes suspension. These samples were filtered (not centrifuged) before taking a spectrum. DataMb/Tris in Acetone, Trial 1
The drop in absorbance at 140 minutes is due to the outside of the cuvette being cleaned. Mb/Tris in Acetone, Trial 2 [[]] The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned. | |
