User:Dhea Patel/Notebook/Hemoglobin Project/2013/06/11: Difference between revisions
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The [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/31 procedure]for the reaction of OPD and H<sub>2</sub>O<sub>2</sub> was followed using a Mb/Tris catalyst and an acetonitrile suspension. Like [[User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/06/10| yesterday]], the reaction was run in a beaker with a stir bar and the solution was filtered before taking a spectrum. | The [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/31 procedure]for the reaction of OPD and H<sub>2</sub>O<sub>2</sub> was followed using a Mb/Tris catalyst and an acetonitrile suspension. Like [[User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/06/10| yesterday]], the reaction was run in a beaker with a stir bar and the solution was filtered before taking a spectrum. | ||
In addition reactions were run using Mb/ | In addition reactions were run using Mb/Phosphate and Mb/Acetate in acetone suspension. | ||
Because the acetone corroded the plastic, samples 80mins-140mins are taken without filtering the sample. | |||
==Data== | ==Data== | ||
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<b>Mb/Tris in Acetonitrile</b> | <b>Mb/Tris in Acetonitrile</b> | ||
[[Image:Mb_Tris1_OPD_H2O2_MeCN_FILTERED_WORKUP_GRAPH.png]] | [[Image:Mb_Tris1_OPD_H2O2_MeCN_FILTERED_WORKUP_GRAPH.png|750px]] | ||
Note: The source was off during the 80mins measurement, so data was not available. | Note: The source was off during the 80mins measurement, so data was not available. | ||
[[]] | [[Image:Mb_Tris2_OPD_H2O2_MeCN_WORKUP_GRAPH.png|750px]] | ||
<b>Mb/Phosphate in Acetone</b> | |||
[[Image:Mb_Phosphate_OPD_H2O2_Acetone_WORKUP_GRAPH.png|750px]] | |||
[[]] | <b>Mb/Acetate in Acetone</b> | ||
[[Image:Mb_Acetate_OPD_H2O2_Acetone_WORKUP_GRAPH.png|750px]] | |||
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Revision as of 09:05, 8 July 2013
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Reaction of OPD+H2O2 with Mb Catalyst in Acetonitrile SuspensionThe procedurefor the reaction of OPD and H2O2 was followed using a Mb/Tris catalyst and an acetonitrile suspension. Like yesterday, the reaction was run in a beaker with a stir bar and the solution was filtered before taking a spectrum. In addition reactions were run using Mb/Phosphate and Mb/Acetate in acetone suspension. Because the acetone corroded the plastic, samples 80mins-140mins are taken without filtering the sample. DataMb/Tris in Acetonitrile
Note: The source was off during the 80mins measurement, so data was not available.
Mb/Acetate in Acetone
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