User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/26: Difference between revisions

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*The data was analyzed and compared to the data from January 30th. To find that the UV-vis absorbance was the same, but the Fluorescence had a greater intensity.  
*The data was analyzed and compared to the data from January 30th. To find that the UV-vis absorbance was the same, but the Fluorescence had a greater intensity.  


*UV-vis from 02/26/2013 and 01/30/2013:
[[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH%2C_taken_February_26%2C_2013.png|500px]] [[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png|500px]]


[[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH%2C_taken_February_26%2C_2013.png]] [[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png]]
*Fluorescence from 02/26/2013 and 01/30/2013:
 
[[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH%2C_taken_February_26%2C_2013.png|500px]] [[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png|500px]]
[[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH%2C_taken_February_26%2C_2013.png]] [[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png]]




Revision as of 08:28, 26 February 2013

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Objective

  • to run UV-vis on 11.6mg p-sorbitol in Citrate Buffer in MeOH at temperatures ranging from 5°C to 50°C, with 5°C increments.

Protocol

  • MeOH blank was run in UV-vis and Fluorescence.
  • 11.6mg of myoglobin with KCl in 20mL 0.01M pH 3 citrate buffer (evaporated) made January 30th was run in UV-vis and Fluorescence.
  • The data was analyzed and compared to the data from January 30th. To find that the UV-vis absorbance was the same, but the Fluorescence had a greater intensity.
  • UV-vis from 02/26/2013 and 01/30/2013:

  • Fluorescence from 02/26/2013 and 01/30/2013:



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