User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/15

From OpenWetWare

< User:Dhea Patel | Notebook | Hemoglobin Project | 2013 | 02(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/02/15 Entry for User:Dhea_Patel/Notebook/Hemoglobin_Project)
Current revision (09:58, 15 February 2013) (view source)
m
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Objective==
-
* Insert content here...
+
* Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.
 +
==Description==
 +
 +
*10.5mg mannitol in Tris buffer, solvent: Acetonitrile
 +
*10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
 +
*10.5mg mannitol in tris buffer, solvent: ethyl acetate
 +
*12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
 +
*11.7mg mannitol in tris buffer, solvent: water
 +
*10.0mg p-sorbitol in phosphate buffer, solvent: water
 +
*11.6mg mannitol in  tris buffer, solvent: chloroform
 +
**centrifuged for an additional 10 minutes
 +
*10.0mg p-sorbitol in phosphate buffer, solvent: chloroform
 +
 +
 +
*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
 +
*Centrifuge settings were as follows:
 +
**13200rpm
 +
**0:16 seconds
 +
**851 rotor
 +
*all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
 +
*Each solvent was used as a blank.
 +
*The data was corrected for the blank and a corrected baseline.
 +
*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Project name Main project page
Previous entry      Next entry

Objective

  • Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.

Description

  • 10.5mg mannitol in Tris buffer, solvent: Acetonitrile
  • 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
  • 10.5mg mannitol in tris buffer, solvent: ethyl acetate
  • 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
  • 11.7mg mannitol in tris buffer, solvent: water
  • 10.0mg p-sorbitol in phosphate buffer, solvent: water
  • 11.6mg mannitol in tris buffer, solvent: chloroform
    • centrifuged for an additional 10 minutes
  • 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform


  • all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.


Personal tools