User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/15

Current revision

Project name

Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.

10.5mg mannitol in Tris buffer, solvent: Acetonitrile
10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
10.5mg mannitol in tris buffer, solvent: ethyl acetate
12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
11.7mg mannitol in tris buffer, solvent: water
10.0mg p-sorbitol in phosphate buffer, solvent: water
11.6mg mannitol in tris buffer, solvent: chloroform
- centrifuged for an additional 10 minutes
10.0mg p-sorbitol in phosphate buffer, solvent: chloroform

all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
Centrifuge settings were as follows:
- 13200rpm
- 0:16 seconds
- 851 rotor
all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
Each solvent was used as a blank.
The data was corrected for the blank and a corrected baseline.
Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.

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-==Entry title==
+==Objective==
-* Insert content here...
+* Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.
+==Description==
+*10.5mg mannitol in Tris buffer, solvent: Acetonitrile
+*10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
+*10.5mg mannitol in tris buffer, solvent: ethyl acetate
+*12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
+*11.7mg mannitol in tris buffer, solvent: water
+*10.0mg p-sorbitol in phosphate buffer, solvent: water
+*11.6mg mannitol in  tris buffer, solvent: chloroform
+**centrifuged for an additional 10 minutes
+*10.0mg p-sorbitol in phosphate buffer, solvent: chloroform
+*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
+*Centrifuge settings were as follows:
+**13200rpm
+**0:16 seconds
+**851 rotor
+*all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
+*Each solvent was used as a blank.
+*The data was corrected for the blank and a corrected baseline.
+*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.
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