User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/13

Objective

• Run UV-vis and Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either MeOH, acetone, acetonitrile, ethyl acetate, water, or chloroform.

Description

• 10.5mg mannitol in Tris buffer, solvent: Acetonitrile
• 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
• 10.5mg mannitol in tris buffer, solvent: ethyl acetate
• 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
• 11.7mg mannitol in tris buffer, solvent: water
• 10.0mg p-sorbitol in phosphate buffer, solvent: water
• 11.6mg mannitol in tris buffer, solvent: chloroform
• 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform

• Each solvent was used as a blank.
• The data was corrected for the blank and a corrected baseline.
• UV-vis scanned from 200-800nm

p-Sorbitol in Phosphate Buffer (after sonication) Data

• UV-vis of p-Sorbitol solid in Acetonitrile

• UV-vis of p-Sorbitol solid in Ethyl Acetate

• UV-vis of p-Sorbitol solid in Water

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• UV-vis of p-Sorbitol solid in Chloroform

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Mannitol in Tris Buffer Data

• UV-vis of Mannitol solid in Acetonitrile

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• UV-vis of Mannitol solid in Ethyl Acetate

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• UV-vis of Mannitol solid in Water

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• UV-vis of Mannitol solid in Chloroform

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