User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/06

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Objective

  • to finish running samples from Friday
  • to run samples of solid (0.2g p-sorbitol , 0.01 Mb, 0.1g PEG in 10mL citrate buffer) made January 29th.

Description

  1. 10.0mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL methanol, centrifuged, and measured
  2. 10.2mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL acetone, centrifuged, and measured
  3. 10.0mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL acetonitrile, centrifuged, and measured
  4. 11.4mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL ethyl acetate, centrifuged, and measured
  5. 9.8mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL water, centrifuged, and measured
  6. 9.5mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL chloroform, centrifuged, and measured
  7. 11.9mg of the "p-sorbitol in Citrate buffer" solid in 1mL acetone was centrifuged, and measured
  8. 9.7mg of the "p-sorbitol in Citrate buffer" solid in 1mL methanol was centrifuged, and measured
  • all the samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • UV-vis scanned from 200-800nm
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.

Data

  • Please see yesterday's Data for the UV-vis and Fluorescence spectra of the p-sorbitol in Citrate samples.


  • UV-vis of "p-sorbitol in Acetate buffer" solid in MeOH

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  • Fluorescence of "p-sorbitol in Acetate buffer" solid in MeOH

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  • UV-vis of "p-sorbitol in Acetate buffer" solid in Acetone

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  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetone

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  • UV-vis of "p-sorbitol in Acetate buffer" solid in Acetonitrile

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  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetonitrile

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  • UV-vis of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate

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  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate

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  • UV-vis of "p-sorbitol in Acetate buffer" solid in Water

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  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Water

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  • UV-vis of "p-sorbitol in Acetate buffer" solid in Chloroform

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  • Fluorescence of "p-sorbitol in Acetate buffer" solid in Chloroform

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