User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/06: Difference between revisions
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== | ==Objective== | ||
* | *to finish running samples from [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/01|Friday]] | ||
*to run samples of solid (0.2g p-sorbitol , 0.01 Mb, 0.1g PEG in 10mL citrate buffer) made January 29th. | |||
==Description== | |||
# 10.0mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL methanol, centrifuged, and measured | |||
# 10.2mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL acetone, centrifuged, and measured | |||
# 10.0mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL acetonitrile, centrifuged, and measured | |||
# 11.4mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL ethyl acetate, centrifuged, and measured | |||
# 9.8mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL water, centrifuged, and measured | |||
# 9.5mg of the "p-sorbitol in Acetate buffer" solid was added to 1mL chloroform, centrifuged, and measured | |||
##the solid remained in a clump and floated to the top of the tube. | |||
# 11.9mg of the "p-sorbitol in Citrate buffer" solid in 1mL acetone was centrifuged, and measured | |||
##as this mixture sat in the fridge over night, the solid began to dissipate and float to the top. | |||
# 9.7mg of the "p-sorbitol in Citrate buffer" solid in 1mL methanol was centrifuged, and measured | |||
*all the samples were vortexed and centrifuged before being measured | |||
* Centrifuge settings were as follows: | |||
**13200rpm | |||
**0:16 seconds | |||
**851 rotor | |||
* Each solvent was used as a blank. | |||
* The data was corrected for the blank and a corrected baseline. | |||
* UV-vis scanned from 200-800nm | |||
* Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min. | |||
=Data= | |||
*Please see [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/05#Data|yesterday's Data]] for the UV-vis and Fluorescence spectra of the p-sorbitol in Citrate samples. | |||
*UV-vis of "p-sorbitol in Acetate buffer" solid in MeOH | |||
[[]] | |||
*Fluorescence of "p-sorbitol in Acetate buffer" solid in MeOH | |||
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*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetone | |||
[[]] | |||
*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetone | |||
[[]] | |||
*UV-vis of "p-sorbitol in Acetate buffer" solid in Acetonitrile | |||
[[]] | |||
*Fluorescence of "p-sorbitol in Acetate buffer" solid in Acetonitrile | |||
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*UV-vis of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate | |||
[[]] | |||
*Fluorescence of "p-sorbitol in Acetate buffer" solid in Ethyl Acetate | |||
[[]] | |||
*UV-vis of "p-sorbitol in Acetate buffer" solid in Water | |||
[[]] | |||
*Fluorescence of "p-sorbitol in Acetate buffer" solid in Water | |||
[[]] | |||
*UV-vis of "p-sorbitol in Acetate buffer" solid in Chloroform | |||
[[]] | |||
*Fluorescence of "p-sorbitol in Acetate buffer" solid in Chloroform | |||
[[]] | |||
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Revision as of 10:11, 6 February 2013
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