User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/05: Difference between revisions
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==Objective== | |||
*to finish running samples from [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/01|Friday]] | |||
*to run samples of solid (0.2g p-sorbitol , 0.01 Mb, 0.1g PEG in 10mL citrate buffer) made January 29th. | |||
==Description== | |||
# 10.0mg of the "Myoglobin in Citrate buffer" solid was added to 1mL water | |||
# 10.2mg of the "Myoglobin in Citrate buffer" solid was added to 1mL chloroform | |||
# 10.0mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL water | |||
# 11.4mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL chloroform | |||
##the solid remained in a clump and floated to the top of the tube. | |||
# 9.8mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL ethyl acetate | |||
# 9.5mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetonitrile | |||
# 11.9mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetone | |||
# 9.7mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL methanol | |||
*all the samples were vortexed and centrifuged before being measured | |||
* Centrifuge settings were as follows: | |||
**13200rpm | |||
**0:16 seconds | |||
**851 rotor | |||
* each solvent was used as a blank. | |||
* The data was corrected for the blank and a corrected baseline. | |||
*UV-vis scanned from 200-800nm | |||
*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min. | |||
=Data= | |||
*Please see [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/01#Data|last Friday's Data]] for the UV-vis and Fluorescence spectra of the myoglobin samples. | |||
*UV-vis of "p-sorbitol in Citrate buffer" solid in MeOH | |||
[[Image:9.7mg_psorbitol_citrate_in_MeOH.png]] | |||
*Fluorescence of "p-sorbitol in Citrate buffer" solid in MeOH | |||
[[Image:Fluorescence_of_9.7mg_p-sorbitol_in_Citrate_Buffer_Solid_in_1mL_MeOH_.png]] | |||
*UV-vis of "p-sorbitol in Citrate buffer" solid in Acetone | |||
[[Image:11.9mg_psorbitol_CitrateB_Solid_in_1mL_Acetone.png]] | |||
*Fluorescence of "p-sorbitol in Citrate buffer" solid in Acetone | |||
[[Image:Fluorescence_of_11.9mg_p-sorbitol_in_Citrate_Buffer_Solid_in_1mL_Acetone.png]] | |||
*UV-vis of "p-sorbitol in Citrate buffer" solid in Acetonitrile | |||
[[Image:9.5mg_psorbitol_CitrateB_Solid_in_1mL_Acetonitrile_.png]] | |||
*Fluorescence of "p-sorbitol in Citrate buffer" solid in Acetonitrile | |||
[[Image:Fluorescence_of_9.5mg_p-sorbitol_in_Citrate_Buffer_Solid_in_1mL_Acetonitrile_.png]] | |||
*UV-vis of "p-sorbitol in Citrate buffer" solid in Ethyl Acetate | |||
[[Image:9.8mg_psorbitol_CitrateB_Solid_in_1mL_Ethyl_Acetate_.png]] | |||
*Fluorescence of "p-sorbitol in Citrate buffer" solid in Ethyl Acetate | |||
[[Image:Fluorescence_of_9.8mg_p-sorbitol_in_Citrate_Buffer_Solid_in_1mL_Ethyl_Acetate_.png]] | |||
*UV-vis of "p-sorbitol in Citrate buffer" solid in Water | |||
[[Image:10.0mg_psorbitol_CitrateB_Solid_in_1mL_Water.png]] | |||
*Fluorescence of "p-sorbitol in Citrate buffer" solid in Water | |||
[[Image:Fluorescence_of_10.0mg_p-sorbitol_in_Citrate_Buffer_Solid_in_1mL_Water_.png]] | |||
*UV-vis of "p-sorbitol in Citrate buffer" solid in Chloroform | |||
[[Image:11.4mg_psorbitol_CitrateB_Solid_in_1mL_Chloroform.png]] | |||
*Fluorescence of "p-sorbitol in Citrate buffer" solid in Chloroform | |||
[[Image:Fluorescence_of_11.4mg_p-sorbitol_CitrateB_Solid_in_1mL_Chloroform.png]] | |||
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Revision as of 09:18, 20 February 2013
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WP:TOC Objective
Description
Data
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