User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/05: Difference between revisions
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==Objective== | ==Objective== | ||
*to finish running samples from [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/01|Friday]] | *to finish running samples from [[User:Dhea_Patel/Notebook/Hemoglobin_Project/2013/02/01|Friday]] | ||
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# 10.0mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL water | # 10.0mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL water | ||
# 11.4mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL chloroform | # 11.4mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL chloroform | ||
##the solid remained in a clump and floated to the top of the tube. | |||
# 9.8mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL ethyl acetate | # 9.8mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL ethyl acetate | ||
# 9.5mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetonitrile | # 9.5mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetonitrile | ||
# 11.9mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetone | # 11.9mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL acetone | ||
# 9.7mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL methanol | # 9.7mg of the "p-sorbitol in Citrate buffer" solid was added to 1mL methanol | ||
*all the samples were vortexed and centrifuged before being measured | |||
* Centrifuge settings were as follows: | |||
**13200rpm | |||
**0:16 seconds | |||
**851 rotor | |||
* each solvent was used as a blank. | * each solvent was used as a blank. | ||
* The data was corrected for the blank and a corrected baseline. | * The data was corrected for the blank and a corrected baseline. |
Revision as of 11:06, 6 February 2013
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