User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/01

Objective

• To run UV-vis and Fluorescence on 0.0050g Myoglobin + 0.4856g KCl in 20mL 0.01M pH 5 acetate buffer (evaporated) in various organic solvents.

Description

1. 9.3mg of the solid (0.0050g Myoglobin + 0.4856g KCl in 20mL 0.01M pH 5 acetate buffer (evaporated)) was added to 1 mL of MeOH.
2. 9.3mg of the solid was added to 1mL of Acetone
3. 10.4mg of the solid was added to 1mL Acetonitrile
4. 9.9mg of the solid was added to 1mL Ethyl Acetate
5. 10.0mg of the solid was measured
6. 10.2mg of the solid was measured

• all the samples were vortexed and centrifuged before being measured
• Centrifuge settings were as follows:
  • 13200rpm
  • 0:16 seconds
  • 851 rotor
• each solvent was used as a blank.
• The data was corrected for the blank and a corrected baseline.

• UV-vis scanned from 200-800nm
• Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100.

Data

• UV-vis of solid in MeOH

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• Fluorescence of solid in MeOH

• UV-vis of solid in Acetone

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• Fluorescence of solid in Acetone

• UV-vis of solid in Acetonitrile

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• Fluorescence of solid in Acetonitrile

• UV-vis of solid in Ethyl Acetate

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• Fluorescence of solid in Ethyl Acetate

• UV-vis of solid in Water

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• Fluorescence of solid in Water

• UV-vis of solid in Chloroform

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• Fluorescence of solid in Chloroform