User:Dhea Patel/Notebook/Hemoglobin Project/2013/01/30: Difference between revisions
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==Objective== | ==Objective== | ||
*To run UV-vis and Fluorescence on 0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH | *To run UV-vis and Fluorescence on 0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 3 citrate buffer (evaporated) in various organic solvents. | ||
==Description== | ==Description== | ||
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# 11.4mg of the solid was added to 1mL chloroform | # 11.4mg of the solid was added to 1mL chloroform | ||
*all the samples were vortexed and centrifuged before being measured | |||
* Centrifuge settings were as follows: | |||
**13200rpm | |||
**0:16 seconds | |||
**851 rotor | |||
*each solvent was used as a blank. | *each solvent was used as a blank. | ||
*The data was corrected for the blank and a corrected baseline. | *The data was corrected for the blank and a corrected baseline. | ||
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*UV-vis of solid in MeOH | *UV-vis of solid in MeOH | ||
[[]] | [[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png]] | ||
*Fluorescence of solid in MeOH | *Fluorescence of solid in MeOH | ||
[[]] | [[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png]] | ||
*UV-vis of solid in Acetone | *UV-vis of solid in Acetone | ||
[[]] | [[Image:UV-vis_of_10.9mg_CitrateB_Solid_in_1mL_Acetone.png]] | ||
*Fluorescence of solid in Acetone | *Fluorescence of solid in Acetone | ||
[[]] | [[Image:Fluorescence_of_10.9mg_Citrate_Solid_in_1mL_Acetone.png]] | ||
*UV-vis of solid in Acetonitrile | *UV-vis of solid in Acetonitrile | ||
[[]] | [[Image:UV-vis_of_10.1mg_CitrateB_Solid_in_1mL_Acetonitrile.png]] | ||
*Fluorescence of solid in Acetonitrile | *Fluorescence of solid in Acetonitrile | ||
[[]] | [[Image:Fluorescence_of_10.1mg_Citrate_Solid_in_1mL_Acetonitrile.png]] | ||
*UV-vis of solid in Ethyl Acetate | *UV-vis of solid in Ethyl Acetate | ||
[[]] | [[Image:UV-vis_of_10.5mg_CitrateB_Solid_in_1mL_Ethyl_Acetate.png]] | ||
*Fluorescence of solid in Ethyl Acetate | *Fluorescence of solid in Ethyl Acetate | ||
[[]] | [[Image:Fluorescence_of_10.5mg_Citrate_Solid_in_1mL_Ethyl_Acetate.png]] | ||
*UV-vis of solid in Water | *UV-vis of solid in Water | ||
[[]] | [[Image:UV-vis_of_10.5mg_CitrateB_Solid_in_1mL_Water.png]] | ||
*Fluorescence of solid in Water | *Fluorescence of solid in Water | ||
[[]] | [[Image:Fluorescence_of_10.5mg_Citrate_Solid_in_1mL_Water.png]] | ||
*UV-vis of solid in Chloroform | *UV-vis of solid in Chloroform | ||
[[]] | [[Image:UV-vis_of_10.0mg_CitrateB_Solid_in_1mL_Chloroform.png]] | ||
*Fluorescence of solid in Chloroform | *Fluorescence of solid in Chloroform | ||
[[]] | [[Image:Fluorescence_of_10.0mg_Citrate_Solid_in_1mL_Chloroform.png]] | ||
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Revision as of 07:40, 12 February 2013
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Objective
Description
Data
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