User:Dhea Patel/Notebook/Hemoglobin Project/2013/01/25

Current revision

Project name

To run UV-vis and Fluorescence on 0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated) in various organic solvents.

10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH.
11.0mg of the solid was added to 1mL of Acetone
11.1mg of the solid was added to 1mL Acetonitrile
11.3mg of the solid was added to 1mL Ethyl Acetate
11.3mg of the solid was added to 1mL water
11.4mg of the solid was added to 1mL chloroform

UV-vis scanned from 200-800nm
Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100.

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 ==Description==
-. 10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH.
+# 10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH.
-. 11.0mg of the solid was added to 1mL of Acetone
+# 11.0mg of the solid was added to 1mL of Acetone
-. 11.1mg of the solid was added to 1mL Acetonitrile
+# 11.1mg of the solid was added to 1mL Acetonitrile
-. 11.3mg of the solid was added to 1mL Ethyl Acetate
+# 11.3mg of the solid was added to 1mL Ethyl Acetate
-. 11.3mg of the solid was added to 1mL water
+# 11.3mg of the solid was added to 1mL water
-. 11.4mg of the solid was added to 1mL chloroform
+# 11.4mg of the solid was added to 1mL chloroform
 *each solvent was used as a blank.
 *The data was corrected for the blank and a corrected baseline.
+*UV-vis scanned from 200-800nm
 *Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100.