User:Dhea Patel/Notebook/Hemoglobin Project/2013/01/25: Difference between revisions
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== | ==Objective== | ||
* | *To run UV-vis and Fluorescence on 0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated) in various organic solvents. | ||
==Description== | |||
# 10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH. | |||
# 11.0mg of the solid was added to 1mL of Acetone | |||
# 11.1mg of the solid was added to 1mL Acetonitrile | |||
# 11.3mg of the solid was added to 1mL Ethyl Acetate | |||
# 11.3mg of the solid was added to 1mL water | |||
# 11.4mg of the solid was added to 1mL chloroform | |||
*each solvent was used as a blank. | |||
*The data was corrected for the blank and a corrected baseline. | |||
*UV-vis scanned from 200-800nm | |||
*Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100. | |||
==Data== | |||
*UV-vis of solid in MeOH | |||
[[Image:UV-vis_of_10.6mg_solid_in_1mL_of_MeOH.png|px10]] | |||
*Fluorescence of solid in MeOH | |||
[[Image:Fluorescence_of_10.6mg_solid_in_1mL_MeOH.png|px10]] | |||
*UV-vis of solid in Acetone | |||
[[Image:UV-vis_of_solid_in_Acetone.png|px10]] | |||
*Fluorescence of solid in Acetone | |||
[[Image:Fluorescence_of_11.0mg_solid_in_1mL_Acetone.png|px10]] | |||
*UV-vis of solid in Acetonitrile | |||
[[Image:UV-vis_of_11.0mg_of_solid_in_1mL_of_Acetonitrile.png|px10]] | |||
*Fluorescence of solid in Acetonitrile | |||
[[Image:Fluorescence_of_11.3mg_solid_in_1mL_Acetonitrile.png|px10]] | |||
*UV-vis of solid in Ethyl Acetate | |||
[[Image:UV-vis_of_solid_in_Ethyl_Acetate.png|px10]] | |||
*Fluorescence of solid in Ethyl Acetate | |||
[[Image:Fluorescence_of_11.3mg_solid_in_1mL_Ethyl_Acetate.png|px10]] | |||
*UV-vis of solid in Water | |||
[[Image:UV-vis of 11.3mg of solid in 1mL of water.png|px10]] | |||
*Fluorescence of solid in Water | |||
[[Image:Fluorescence_of_11.3mg_solid_in_1mL_Water.png|px10]] | |||
*UV-vis of solid in Chloroform | |||
[[Image:UV-vis_of_solid_in_Chloroform.png|px10]] | |||
*Fluorescence of solid in Chloroform | |||
[[Image:Fluorescence_of_11.4mg_solid_in_1mL_Chloroform.png|px10]] | |||
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Revision as of 08:22, 1 February 2013
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Objective
Description
Data
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