User:Dhea Patel/Notebook/Hemoglobin Project/2013/01/25: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Description== | ==Description== | ||
# 10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH. | |||
# 11.0mg of the solid was added to 1mL of Acetone | |||
# 11.1mg of the solid was added to 1mL Acetonitrile | |||
# 11.3mg of the solid was added to 1mL Ethyl Acetate | |||
# 11.3mg of the solid was added to 1mL water | |||
# 11.4mg of the solid was added to 1mL chloroform | |||
*each solvent was used as a blank. | *each solvent was used as a blank. | ||
*The data was corrected for the blank and a corrected baseline. | *The data was corrected for the blank and a corrected baseline. | ||
*UV-vis scanned from 200-800nm | |||
*Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100. | |||
==Data== | ==Data== | ||
*UV-vis of solid in MeOH | *UV-vis of solid in MeOH | ||
[[Image:UV-vis_of_10.6mg_solid_in_1mL_of_MeOH.png| | [[Image:UV-vis_of_10.6mg_solid_in_1mL_of_MeOH.png|px10]] | ||
*Fluorescence of solid in MeOH | *Fluorescence of solid in MeOH | ||
[[Image:Fluorescence_of_10.6mg_solid_in_1mL_MeOH.png| | [[Image:Fluorescence_of_10.6mg_solid_in_1mL_MeOH.png|px10]] | ||
*UV-vis of solid in Acetone | *UV-vis of solid in Acetone | ||
[[Image:UV-vis_of_solid_in_Acetone.png| | [[Image:UV-vis_of_solid_in_Acetone.png|px10]] | ||
*Fluorescence of solid in Acetone | *Fluorescence of solid in Acetone | ||
[[Image:Fluorescence_of_11.0mg_solid_in_1mL_Acetone.png| | [[Image:Fluorescence_of_11.0mg_solid_in_1mL_Acetone.png|px10]] | ||
*UV-vis of solid in Acetonitrile | *UV-vis of solid in Acetonitrile | ||
[[Image:UV-vis_of_11.0mg_of_solid_in_1mL_of_Acetonitrile.png| | [[Image:UV-vis_of_11.0mg_of_solid_in_1mL_of_Acetonitrile.png|px10]] | ||
*Fluorescence of solid in Acetonitrile | *Fluorescence of solid in Acetonitrile | ||
[[Image:Fluorescence_of_11.3mg_solid_in_1mL_Acetonitrile.png| | [[Image:Fluorescence_of_11.3mg_solid_in_1mL_Acetonitrile.png|px10]] | ||
*UV-vis of solid in Ethyl Acetate | *UV-vis of solid in Ethyl Acetate | ||
[[Image:UV-vis_of_solid_in_Ethyl_Acetate.png| | [[Image:UV-vis_of_solid_in_Ethyl_Acetate.png|px10]] | ||
*Fluorescence of solid in Ethyl Acetate | *Fluorescence of solid in Ethyl Acetate | ||
[[Image:Fluorescence_of_11.3mg_solid_in_1mL_Ethyl_Acetate.png| | [[Image:Fluorescence_of_11.3mg_solid_in_1mL_Ethyl_Acetate.png|px10]] | ||
*UV-vis of solid in Water | *UV-vis of solid in Water | ||
[[Image:UV-vis of 11.3mg of solid in 1mL of water.png| | [[Image:UV-vis of 11.3mg of solid in 1mL of water.png|px10]] | ||
*Fluorescence of solid in Water | *Fluorescence of solid in Water | ||
[[Image:Fluorescence_of_11.3mg_solid_in_1mL_Water.png| | [[Image:Fluorescence_of_11.3mg_solid_in_1mL_Water.png|px10]] | ||
*UV-vis of solid in Chloroform | *UV-vis of solid in Chloroform | ||
[[Image:UV-vis_of_solid_in_Chloroform.png| | [[Image:UV-vis_of_solid_in_Chloroform.png|px10]] | ||
*Fluorescence of solid in Chloroform | *Fluorescence of solid in Chloroform | ||
[[Image:Fluorescence_of_11.4mg_solid_in_1mL_Chloroform.png| | [[Image:Fluorescence_of_11.4mg_solid_in_1mL_Chloroform.png|px10]] | ||
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