# User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23

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 Revision as of 16:20, 23 January 2013 (view source) (→Experimental Protocol)← Previous diff Revision as of 16:21, 23 January 2013 (view source) (→Experimental Protocol)Next diff → Line 39: Line 39: *To prepare a 30 mM stock solution of adenosine: *To prepare a 30 mM stock solution of adenosine: - $\frac{0.030mol}{L}$ of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172g adenosine}{L buffer} + [itex]\frac{0.030mol}{L}$ of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172g}{L}$ of adenosine in buffer

## Revision as of 16:21, 23 January 2013

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## Objective

• To complete project proposal
• To complete experimental protocol for next week.

## Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

• Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
• The pH of the final solution will be 7.4.
• This buffer can be stored for up to 1 mo at 4°C.
• Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

• The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

• To prepare a 30 mM stock solution of adenosine:

$\frac{0.030mol}{L}$ of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172g}{L}$ of adenosine in buffer