User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23

(Difference between revisions)

Revision as of 16:15, 23 January 2013

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
- The pH of the final solution will be 7.4.
- This buffer can be stored for up to 1 mo at 4°C.
Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

@@ Line 24: / Line 24: @@
 ==Experimental Protocol==
-'''Preparing Phosphate Buffer'''
+'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
+*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
+**The pH of the final solution will be 7.4.
+**This buffer can be stored for up to 1 mo at 4°C.
+*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
 '''Running ADA Kinetics'''