User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23

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(Experimental Protocol)
Current revision (16:24, 23 January 2013) (view source)
 
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*To prepare a 30 mM stock solution of adenosine:  
*To prepare a 30 mM stock solution of adenosine:  
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.030  <math>\frac{mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math>
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<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g}{L}</math> of adenosine in buffer
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*To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.
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Objective

  • To complete project proposal
  • To complete experimental protocol for next week.


Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

px80

  • To prepare a 30 mM stock solution of adenosine:

\frac{0.030mol}{L} of adenosine × \frac{267.24  g}{1  mol} = \frac{8.0172g}{L} of adenosine in buffer

  • To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.



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