Dhea Patel at 19:38, 13 November 2012

2012-11-13T19:38:51Z

←Older revision		Revision as of 19:38, 13 November 2012
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	==Objective==		==Objective==

-	~~#To make biocompatible protein films with specific reactive sites~~	+	*to run Bradford Assays on the purified fractions of ADA from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/03\|October]] and [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/06\| November]]
-	~~#Explore~~ the ~~solution conditions (pH, ionic strength, buffer, stabilizers) that produce stable protein – gold nanoparticle suspensions~~	+	*to prepare AuHRP solutions

		+	==Description==
		+
		+	*Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/11/07\|Melissa's entry]] for details regarding the Bradford Assay on purified ADA protein.
		+	**Diluted Bradford solution was reacted with samples of purified ADA protein fractions. UV-vis was run on the samples and the data was plotted in Excel.
		+
		+	*In order to determine which Au/HRP concentrations were required to form aggregates, Au/HRP solutions were made with molar ratios of 10, 50, 100, 150, 200, 250, 300, 350, 400, and 450. The image below details the calculations and volumes used to create the solutions.
		+
		+	[[Image:20121106_AuHRP_Calculations.png]]
		+
		+	*After the solutions were made, the samples were wrapped in aluminum foil and placed in the oven at 80°C for 4 hours.
		+
		+	==Notes==
		+
		+	*The HAuCl<sub>4</sub> stock solution from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/05\|September 5th]] was used to create the Au/HRP solutions.

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Dhea Patel: Autocreate 2012/11/07 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook

2012-11-07T17:29:10Z

Autocreate 2012/11/07 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook

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==Objective==

#To make biocompatible protein films with specific reactive sites
#Explore the solution conditions (pH, ionic strength, buffer, stabilizers) that produce stable protein – gold nanoparticle suspensions


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User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/07 - Revision history

Dhea Patel at 19:38, 13 November 2012

Dhea Patel: Autocreate 2012/11/07 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook