User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/06

Objective

• to run transformations of PCR product into E. coli cells
• to run FPLC on the ADA protein from the third and the fourth of November.

Description

• The cells from the fourth of November were sonciated for 30 seconds and then placed in an ice bath for 30 seconds. This was repeated for a total of 3 times.
• The cells were then transferred to centrifuge tubes, balanced, and placed in the centrifuge at 18,000rpm at 4°C for 2 hours.
• The cells were then filtered using...
• The cells were then run on FPLC, using the binding buffer and elusion buffer from September 26th.
• The cells were collected in 5 mL aliquots and transferred into a Falcon tube.

• The Au/Lysozyme solutions were obtained from the oven. The following images are the results from the Au/Lysozyme reaction.
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