User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/03

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(Autocreate 2012/11/03 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
Current revision (20:21, 4 November 2012) (view source)
 
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==Objective==
==Objective==
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#To make biocompatible protein films with specific reactive sites
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*to amplify ADA protein in LB media
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#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
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==Description==
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Please refer to [[User:Keyun_Wang/Notebook/Experimental_Biological_Chemistry_I/2012/11/03| Kay's entry]] for the protocol and details of:
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*creating 4 sets of 35mL and 4 sets of 1L LB media,
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*autoclaving the media,
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*inoculating the media,
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*adding Kanamycin,
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*and creating the starter culture in the four 35mL LB media.
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Objective

  • to amplify ADA protein in LB media

Description

Please refer to Kay's entry for the protocol and details of:

  • creating 4 sets of 35mL and 4 sets of 1L LB media,
  • autoclaving the media,
  • inoculating the media,
  • adding Kanamycin,
  • and creating the starter culture in the four 35mL LB media.


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