User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/31

(Difference between revisions)

Revision as of 21:15, 4 November 2012

to create Au/Lysozyme solutions using the protocol used to make Au/BSA solutions.

Solutions of Au/Lysozyme were created, using the HAuCl₄ solutions made on September 5th.
70uM stock solution of Lysozyme was made by adding 5mL H₂ to 0.005g of Lysozyme.
The following table indicates the volumes of lysozyme, HAuCl₄, and water used in order to create 20, 30, 40, 50, 60, 70, 80, 100, 120, and 130 uM rations of Au/Lysozyme.

The test tubes were labeled with the mixture's ratios, covered in foil, capped, and placed in the oven at 80°C for 4 hours.

@@ Line 17: / Line 17: @@
 ==Objective==
-#To make biocompatible protein films with specific reactive sites
+*to create Au/Lysozyme solutions using the protocol used to make Au/BSA solutions.
-#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
+==Description==
+#Solutions of Au/Lysozyme were created, using the HAuCl<sub>4</sub> solutions made [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/05| on September 5th]].
+#70uM stock solution of Lysozyme was made by adding 5mL H<sub>2</sub> to 0.005g of Lysozyme.
+#The following table indicates the volumes of lysozyme, HAuCl<sub>4</sub>, and water used in order to create 20, 30, 40, 50, 60, 70, 80, 100, 120, and 130 uM rations of Au/Lysozyme.
+[[Image:Au_lysozyme_solutions.png]]
+#The test tubes were labeled with the mixture's ratios, covered in foil, capped, and placed in the oven at 80°C for 4 hours.
+[[Image:Au_lysozyme_solutions.png]]
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