User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/23

Objective

• to create protein fibers in lysozyme/Au solutions
• to prepare solutions for chemiluminescence reactions

Description

Lysozyme/Au Solutions

• HAuCl₄ and Lysozyme solutions were prepared.
  • 7.4mg HAuCl₄ in 5.0 mL --> 4.36 mM
  • 0.001g Lysozyme in 5.0 mL -->5mM
  • 8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles by Yeonju Lee and Kurt E. Geckeler.
    • The solutions bubbled over in the microwave. No fibers were formed. Next time, will try the same protocol as used when creating the Au/BSA mixtures in September.

Preparing Solutions for Chemilluminescence

• 0.564 mM luminol stock solution was prepared in carbonate buffer
  • 0.0010 g luminol + 10 mL carbonate buffer --> 0.564 mM luminol solution.
  • Goal: 0.5mM solution
• 5 mM H₂O₂ stock solution in carbonate buffer:
  • 3.9 μL of 30 wt% H₂O₂ + 10 mL of carbonate buffer --> 5 mM H₂O₂solution.
• 9.2 μM HRP solution
  • 4.0 mg HRP + 1 mL autoclaved water --> 9.2 μM HRP solution.