User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/23

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''Lysozyme/Au Solutions''
''Lysozyme/Au Solutions''
*HAuCl<sub>4</sub> and Lysozyme solutions were prepared.  
*HAuCl<sub>4</sub> and Lysozyme solutions were prepared.  
-
**7.4mg  HAuCl<sub>4</sub> in 5.0 mL -->  
+
**7.4mg  HAuCl<sub>4</sub> in 5.0 mL --> 4.36 mM
**0.001g Lysozyme in 5.0 mL -->5mM
**0.001g Lysozyme in 5.0 mL -->5mM
**8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper ''Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles'' by Yeonju Lee and Kurt E. Geckeler.
**8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper ''Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles'' by Yeonju Lee and Kurt E. Geckeler.

Revision as of 22:50, 29 November 2012

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Objective

  • to create protein fibers in lysozyme/Au solutions
  • to prepare solutions for chemiluminescence reactions

Description

Lysozyme/Au Solutions

  • HAuCl4 and Lysozyme solutions were prepared.
    • 7.4mg HAuCl4 in 5.0 mL --> 4.36 mM
    • 0.001g Lysozyme in 5.0 mL -->5mM
    • 8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles by Yeonju Lee and Kurt E. Geckeler.
      • The solutions bubbled over in the microwave. No fibers were formed. Next time, will try the same protocol as used when creating the Au/BSA mixtures in September.


Preparing Solutions for Chemilluminescence

  • 0.564 mM luminol stock solution was prepared in carbonate buffer
    • 0.0010 g luminol + 10 mL carbonate buffer --> 0.564 mM luminol solution.
    • Goal: 0.5mM solution
  • 5 mM H2O2 stock solution in carbonate buffer:
    • 3.9 μL of 30 wt% H2O2 + 10 mL of carbonate buffer --> 5 mM H2O2solution.
  • 9.2 μM HRP solution
    • 4.0 mg HRP + 1 mL autoclaved water --> 9.2 μM HRP solution.


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