User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/23

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**0.001g Lysozyme in 5.0 mL -->5mM
**0.001g Lysozyme in 5.0 mL -->5mM
**8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper ''Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles'' by Yeonju Lee and Kurt E. Geckeler.
**8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper ''Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles'' by Yeonju Lee and Kurt E. Geckeler.
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***The solutions bubbled over in the microwave. No fibers were formed. Next time, will try the same protocol as used when creating the Au/BSA mixtures in September.
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Revision as of 09:15, 25 October 2012

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Objective

  • to create protein fibers in lysozyme/Au solutions
  • to prepare solutions for chemiluminescence reactions

Description

  • HAuCl4 and Lysozyme solutions were prepared.
    • 7.4mg HAuCl4 in 5.0 mL -->
    • 0.001g Lysozyme in 5.0 mL -->5mM
    • 8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles by Yeonju Lee and Kurt E. Geckeler.
      • The solutions bubbled over in the microwave. No fibers were formed. Next time, will try the same protocol as used when creating the Au/BSA mixtures in September.


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