User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/23

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(Autocreate 2012/10/23 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
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==Objective==
==Objective==
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#To make biocompatible protein films with specific reactive sites
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*to create protein fibers in lysozyme/Au solutions
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#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
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*to prepare solutions for chemiluminescence reactions
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==Description==
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*HAuCl<sub>4</sub> and Lysozyme solutions were prepared.
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**7.4mg  HAuCl<sub>4</sub> in 5.0 mL -->
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**0.001g Lysozyme in 5.0 mL -->5mM
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**8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper ''Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles'' by Yeonju Lee and Kurt E. Geckeler.
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Revision as of 10:14, 25 October 2012

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Objective

  • to create protein fibers in lysozyme/Au solutions
  • to prepare solutions for chemiluminescence reactions

Description

  • HAuCl4 and Lysozyme solutions were prepared.
    • 7.4mg HAuCl4 in 5.0 mL -->
    • 0.001g Lysozyme in 5.0 mL -->5mM
    • 8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles by Yeonju Lee and Kurt E. Geckeler.


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